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…a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years. —Pharmaceutical Research This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.

Annual Plant Reviews, Volume 12 A fundamental feature of developmental biology is that of the establishment of polarity. It can be described at different levels – polarity of the organism, polarity in tissue patterning and organ development, and polarity of the cell. This volume provides an account of current research into the mechanisms by which polarity is generated at the level of the cell, organ and organism in plants, drawing especially on recent work with model organisms.The emphasis is on the use of the techniques of molecular genetics to dissect molecular mechanisms. This is the first volume to bring together the diverse aspects of polarity in plant development. It is directed at researchers and professionals in plant developmental biology, cell biology and molecular biology. Visit www.blackwellplantsci.com the plant science site from Blackwell Publishing.

Cl- absorption and HCO3- secretion are intimately associated processes vital to epithelial function, itself a key physiological activity. Until recently the transporters responsible remained obscure, but a breakthrough occurred with the discovery of the SLC26 transporters family. It is now clear that the SLC26 transporters have broad physiological functions since mutations in several members are linked to a variety of diseases. This book describes the properties of this family in detail, with contributions from the leading global researchers in the field. Complementary views from experts on other ion channels are offered in the discussions, which make fascinating reading. This family consists of at least 10 genes, each of which has several splice variants. Most members of the family are expressed in the luminal membrane of epithelial cells. Characterization of anion transport by three members has revealed that all function as Cl-/HCO3- exchangers, suggesting that SLC26 transporters are responsible for the luminal Cl-/HCO3- exchange activity. The SLC26 transporters are activated by the CF transmembrane conductance regulator and activate it in turn, leading to a model in which these molecules act together to mediate epithelial Cl- absorption and HCO3- secretion. The book includes chapters on the transport of other molecules by the SLC26 family, including oxalate in the kidney and sugars in cochlear hair cells amongst others. It also describes recent discoveries that most SLC26 transporters bind to scaffold proteins and that they all contain a conserved domain predicted to participate in protein-protein interactions. These suggest the SLC26 transporters exist in complexes with other Cl- and HCO3- transporters, and possibly other regulatory proteins. This book explores the functional role of these interactions, leading to better understanding of transepithelial fluid and electrolyte secretion and the diseases associated with it.

Epithelial cells cover the outer and inner surfaces of the body, forming a selective polarized barrier between the intercellualar space and the 'external' world. Linking the cells of this continuous layer and contributing to epithelial organization and function are specialized membrane domains–desmosomes, gap junctions, and occluding junctions. The contributors to this multidisciplinary symposium volume explore the nature of such junctional structures, focusing on the molecular organization and diversity of their constituent proteins, their formation and control, and interactions with ions and cytoskeletal elements. The physiological significance of cell-cell interaction in epithelia is considered, with reference to cell adhesion, barrier formation and intercellular communication, and to the functional implications for tissue architecture, embryonic development, morphogenesis and carcinogenesis.

Precise and Efficient
Polarity analysis is an efficient method that helps a busy practitioner by making the homeopathic prescription faster and more precise. The Swiss physician Heiner Frei developed this method to demonstrate the efficacy of homeopathic treatment of ADHD children in a controlled 5-year clinical study. The study demonstrated highly significant effects of homeopathy.
Polarity analysis is based on Boenninghausen’s Therapeutic Pocketbook and has revolutionised homeopathic treatment. Cornerstones of the prescription are polar symptoms such as amelioration or aggravation by heat or motion. They mirror the disturbed vital force. Polarity analysis goes directly to the core of the case. It offers clear differentiation of a manageable number of 133 remedies.
Heiner Frei’s method is easy to learn. He shows us all its facets and nuances by leading us through 40 exciting cases, from acute hearing loss, allergic disease, chronic obstructive bronchitis, mononucleosis, mumps and scarlet fever to ADHD, Asperger syndrome and epilepsy. Casetaking is facilitated by checklists and questionnaires.
This book comes from the large practice of an experienced homeopathic physician as well as from a passionate researcher who will not rest until he can bring his inspired discovery to perfection. His success rate of 80% speaks for itself.
“With Heiner Frei’s polarity analysis, the Boenninghausen method has reached a peak of perfection which we have witnessed in our practice. The modalities are a direct reaction of the vital force to any pathological disturbance: unlike psychological symptoms, they do not require any interpretation. Polarity analysis is a reliable method and it enhances our work, especially for quick treatment of acute illness and for hyperactive children – we would certainly not want to do without it again.” Ulrich Welte
"By the way, the book is beyond fantastic. It is without a doubt the clearest, most precise homeopathy book I have read. Congrats over and again!" Lauren Hubele
"I bought the book and love it. Read it cover to cover twice in a week." Peter Gold